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Hi gang,

Many of you asked to be kept posted regarding the research study at Emory.

Well, here's an update: I can't get through to a real person!! I have left

numerous voice mails but haven't been called back. So I'm a tad bit

frustrated right now.

Regarding Klonopin and memory loss, thanks for all the suggestions on how

to possibly deal with this situation. I need to compile a list and evaluate

the whole thing, if I can remember to do it! Just kidding - I'm not that

bad, at least on good days. :) Several of you suggested I stop taking the

Klonopin, but I feel very torn about that idea. I can either keep on the

med and at least sleep 5 hours or so, thus keeping my back from acting up.

Or I can quit Klonopin, hopefully have better memory function, yet take the

chance of having my muscle spasms start back (happens whenever I don't

adequately rest my back). I just don't know.

Anyway, thanks for all the response I've gotten and I will continue to keep

you posted if anything develops with this study. Meanwhile, try to have

sweet dreams!

, Atlanta, 34

the city that has everyone doing the " dirty bird "

GO FALCONS

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  • 10 years later...
Guest guest

Desr ;

I am curious if Drs. Bowlus and Gershwin will be looking at the balance between

Th17 and Treg cells in this study? There is growing evidence that this

(im)balance is important in IBD and PBC; see for example:

__________________________________

Clinical & Experimental Immunology Published Online: 4 Feb 2009

Imbalance between T helper type 17 and T regulatory cells in patients with

primary biliary cirrhosis: the serum cytokine profile and peripheral cell

population.

G. Rong,* § Y. Zhou,* § Y. Xiong,* L. Zhou,* H. Geng,* T. Jiang,* Y. Zhu,* H.

Lu,* S. Zhang,* P. Wang, † B. Zhang ‡ and R. Zhong*

*Department of Laboratory Medicine, Changzheng Hospital, and Departments of

† Gastroenterology, and ‡ General Surgery, Changhai Hospital, Second Military

Medical University, Shanghai, China

Correspondence to R. Zhong, Department of Laboratory Medicine, Changzheng

Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai,

200003, China.

E-mail: rqzhong@...

§ Guanghua Rong and Yunheng Zhou contributed equally to this work.

KEYWORDS

autoimmunity • PBC • Th17 cells • Treg cells

ABSTRACT

Primary biliary cirrhosis (PBC) is an organ-specific autoimmune liver disease

characterized by progressive loss of intrahepatic small bile ducts. Cellular

immune mechanisms involving T cell reaction are thought to be involved

significantly in the pathogenesis of PBC. Recent studies have independently

revealed enhanced T helper type 17 (Th17) response and weakened T regulatory

cell (Treg) response in some autoimmune diseases, indicating a role of Th17/Treg

imbalance in the pathogenesis of autoimmunity. This prompted us to investigate

whether the Th17/Treg balance was broken in the peripheral blood of patients

with PBC and, if it was, what cytokine circumstances might contribute to this

imbalance. The expression of 11 Th17/Treg differentiation-related genes and

serum concentrations of the corresponding cytokines in 36 patients with PBC, 28

patients with chronic hepatitis B and 28 healthy controls were measured by

real-time quantitative-polymerase chain reaction and enzyme-linked immunosorbent

assay respectively. Peripheral Th17 and Treg cells were analysed by flow

cytometry. Th17-related cytokines were increased significantly in patients with

PBC. Consistent with the cytokine profile, the Th17 cell population and

retinoid-related orphan receptor gammat expression were increased markedly. In

contrast, the Treg cell population and forkhead box P3 expression were decreased

dramatically in the peripheral blood of patients with PBC. Our study revealed

that the Th17/Treg imbalance, both cytokine profile and cell numbers, exists in

patients with PBC, suggesting its potential role in the breakdown of immune

self-tolerance in PBC. Interleukin-23, which characterized the imbalanced

cytokine profile, may play an essential role in Th17-related human autoimmunity.

__________________________________

So it would seem reasonble to look to see if this balance is also disturbed in

PSC. I couldn't tell from your post what receptor they are looking for, and in

which type of T cell.

Best regards,

Dave

(father of (23); PSC 07/03; UC 08/03)

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Guest guest

Desr ;

I am curious if Drs. Bowlus and Gershwin will be looking at the balance between

Th17 and Treg cells in this study? There is growing evidence that this

(im)balance is important in IBD and PBC; see for example:

__________________________________

Clinical & Experimental Immunology Published Online: 4 Feb 2009

Imbalance between T helper type 17 and T regulatory cells in patients with

primary biliary cirrhosis: the serum cytokine profile and peripheral cell

population.

G. Rong,* § Y. Zhou,* § Y. Xiong,* L. Zhou,* H. Geng,* T. Jiang,* Y. Zhu,* H.

Lu,* S. Zhang,* P. Wang, † B. Zhang ‡ and R. Zhong*

*Department of Laboratory Medicine, Changzheng Hospital, and Departments of

† Gastroenterology, and ‡ General Surgery, Changhai Hospital, Second Military

Medical University, Shanghai, China

Correspondence to R. Zhong, Department of Laboratory Medicine, Changzheng

Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai,

200003, China.

E-mail: rqzhong@...

§ Guanghua Rong and Yunheng Zhou contributed equally to this work.

KEYWORDS

autoimmunity • PBC • Th17 cells • Treg cells

ABSTRACT

Primary biliary cirrhosis (PBC) is an organ-specific autoimmune liver disease

characterized by progressive loss of intrahepatic small bile ducts. Cellular

immune mechanisms involving T cell reaction are thought to be involved

significantly in the pathogenesis of PBC. Recent studies have independently

revealed enhanced T helper type 17 (Th17) response and weakened T regulatory

cell (Treg) response in some autoimmune diseases, indicating a role of Th17/Treg

imbalance in the pathogenesis of autoimmunity. This prompted us to investigate

whether the Th17/Treg balance was broken in the peripheral blood of patients

with PBC and, if it was, what cytokine circumstances might contribute to this

imbalance. The expression of 11 Th17/Treg differentiation-related genes and

serum concentrations of the corresponding cytokines in 36 patients with PBC, 28

patients with chronic hepatitis B and 28 healthy controls were measured by

real-time quantitative-polymerase chain reaction and enzyme-linked immunosorbent

assay respectively. Peripheral Th17 and Treg cells were analysed by flow

cytometry. Th17-related cytokines were increased significantly in patients with

PBC. Consistent with the cytokine profile, the Th17 cell population and

retinoid-related orphan receptor gammat expression were increased markedly. In

contrast, the Treg cell population and forkhead box P3 expression were decreased

dramatically in the peripheral blood of patients with PBC. Our study revealed

that the Th17/Treg imbalance, both cytokine profile and cell numbers, exists in

patients with PBC, suggesting its potential role in the breakdown of immune

self-tolerance in PBC. Interleukin-23, which characterized the imbalanced

cytokine profile, may play an essential role in Th17-related human autoimmunity.

__________________________________

So it would seem reasonble to look to see if this balance is also disturbed in

PSC. I couldn't tell from your post what receptor they are looking for, and in

which type of T cell.

Best regards,

Dave

(father of (23); PSC 07/03; UC 08/03)

Link to comment
Share on other sites

Guest guest

Desr ;

I am curious if Drs. Bowlus and Gershwin will be looking at the balance between

Th17 and Treg cells in this study? There is growing evidence that this

(im)balance is important in IBD and PBC; see for example:

__________________________________

Clinical & Experimental Immunology Published Online: 4 Feb 2009

Imbalance between T helper type 17 and T regulatory cells in patients with

primary biliary cirrhosis: the serum cytokine profile and peripheral cell

population.

G. Rong,* § Y. Zhou,* § Y. Xiong,* L. Zhou,* H. Geng,* T. Jiang,* Y. Zhu,* H.

Lu,* S. Zhang,* P. Wang, † B. Zhang ‡ and R. Zhong*

*Department of Laboratory Medicine, Changzheng Hospital, and Departments of

† Gastroenterology, and ‡ General Surgery, Changhai Hospital, Second Military

Medical University, Shanghai, China

Correspondence to R. Zhong, Department of Laboratory Medicine, Changzheng

Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai,

200003, China.

E-mail: rqzhong@...

§ Guanghua Rong and Yunheng Zhou contributed equally to this work.

KEYWORDS

autoimmunity • PBC • Th17 cells • Treg cells

ABSTRACT

Primary biliary cirrhosis (PBC) is an organ-specific autoimmune liver disease

characterized by progressive loss of intrahepatic small bile ducts. Cellular

immune mechanisms involving T cell reaction are thought to be involved

significantly in the pathogenesis of PBC. Recent studies have independently

revealed enhanced T helper type 17 (Th17) response and weakened T regulatory

cell (Treg) response in some autoimmune diseases, indicating a role of Th17/Treg

imbalance in the pathogenesis of autoimmunity. This prompted us to investigate

whether the Th17/Treg balance was broken in the peripheral blood of patients

with PBC and, if it was, what cytokine circumstances might contribute to this

imbalance. The expression of 11 Th17/Treg differentiation-related genes and

serum concentrations of the corresponding cytokines in 36 patients with PBC, 28

patients with chronic hepatitis B and 28 healthy controls were measured by

real-time quantitative-polymerase chain reaction and enzyme-linked immunosorbent

assay respectively. Peripheral Th17 and Treg cells were analysed by flow

cytometry. Th17-related cytokines were increased significantly in patients with

PBC. Consistent with the cytokine profile, the Th17 cell population and

retinoid-related orphan receptor gammat expression were increased markedly. In

contrast, the Treg cell population and forkhead box P3 expression were decreased

dramatically in the peripheral blood of patients with PBC. Our study revealed

that the Th17/Treg imbalance, both cytokine profile and cell numbers, exists in

patients with PBC, suggesting its potential role in the breakdown of immune

self-tolerance in PBC. Interleukin-23, which characterized the imbalanced

cytokine profile, may play an essential role in Th17-related human autoimmunity.

__________________________________

So it would seem reasonble to look to see if this balance is also disturbed in

PSC. I couldn't tell from your post what receptor they are looking for, and in

which type of T cell.

Best regards,

Dave

(father of (23); PSC 07/03; UC 08/03)

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