Re: Re: Identification and dynamics of proteins adhering to the surface of medical silicones in vivo and in vitro

[Guest guest]

I dont, , but I emailed and asked

her for it if she still has it, so we will see........I will

let ya know....

Dede

When the power of love

overcomes the love of power

the world will know peace.

~ Jimi Hendrix

Re: Identification and dynamics of proteins adhering to the surface of medical silicones in vivo and in vitro

Thank you, thank you.

Do you have a website/link for this? I tried to track it down on PubMed, but I'm no good at it.

M.

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> From one of our " Sisters "

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> Always a good one to give your doctors...

> so they can be informed tooo....

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> 1.

> J Proteome Res. 2007 Jan;6(1):376-81.

> Identification and dynamics of proteins adhering to the surface of medical silicones in vivo and in vitro.

> Backovic A, Huang HL, Del Frari B, Piza H, Huber LA, Wick G.

> Division for Experimental Pathophysiology and Immunology, Biocenter, Innsbruck Medical University, Fritz-Pregl Str 3/IV, A6020 Innsbruck, Austria.

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> Abstract

> Silicone has been used in medical practice as a paradigmatic implant material for decades despite significant detrimental side effects. Our targeted proteomics approach was aimed at identification of the proteins adsorbed to the surface of silicone because they have been characterized as key components in the onset and perpetuation of local immune reactions to silicone. The composition of the proteinacious film, the dynamics of protein deposition, and protein modifications after adsorption were analyzed both in vivo and in vitro. Differential analysis of protein deposition was performed, followed by protein identification with mass spectrometry, database matching, and Western blots. Thus far, we have identified the 30 most abundant proteins deposited on the surface of silicone, the largest known inventory of such proteins so far. Structural and extracellular matrix proteins predominated, followed by mediators of host defense, metabolism, transport, and stress related pro teins. In addition, several biochemical modifications of fibronectin, vitronectin, and heat shock protein 60 were detected. Our analyses also revealed previously undetected proteins deposited on the surface of silicone. As tentative initiators and/or modulators of the response to silicone, they are therefore valuable candidates for prognosis and therapy.

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> Free Article

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> PMID: 17203981 [PubMed - indexed for MEDLINE]

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