Cystic fibrosis females have a worse prognosis

[Guest guest]

Hi,

I hope that Fiona and all the other girls on the list will prove

these researchers wrong, like n Rojas and Shores

already do :-)))

Please read the abstract with a large grain of salt. After all, our

daughters aren't mice (only rug rats), LOL!

Peace

Torsten

Immunology

Volume 107 Issue 3 Page 297 - November 2002

Influence of gender and interleukin-10 deficiency on the inflammatory

response during lung infection with Pseudomonas aeruginosa in mice

Claudine Guilbault*, Stotland*, Claude Lachance*, Mifong Tam*,

Keller*, Luann -Snipes, Cowley, A.

Hamilton, H. Eidelman, M. son* & Danuta Radzioch*

Summary

Cystic fibrosis females have a worse prognosis compared to male

patients. Furthermore, cystic fibrosis patients infected with

Pseudomonas aeruginosa have been shown to have dysregulated cytokine

profiles, as higher levels of tumour necrosis factor alpha (TNF-),

interleukin (IL)-8, and lower levels of IL-10 are found in the

bronchoalveolar lavage fluid compared to healthy controls. The present

study was aimed at investigating the importance of gender and IL-10 in

the susceptibility of C57BL/6 mice to pulmonary infection with

Pseudomonas aeruginosa. We found that wildtype females were more

susceptible than males to infection, as we observed greater weight

loss,

higher bacterial load, and inflammatory mediators in their lungs. IL-

10

knockout mice, both females and males, had higher levels of TNF- in

the

lungs compared to wildtype mice and maintained higher levels of

polymorphonuclear cells and lower levels of macrophages for a longer

period of time. Our results demonstrate that the number of bacteria

recovered from the lungs of IL-10 knockout male mice was significantly

higher than that observed in their wildtype male counterparts and we

show that neutralization of IL-10 in infected female mice for a

prolonged period of time leads to increased susceptibility to

infection.

Results reported in this study clearly demonstrate that females, both

wildtype and IL-10 knockout mice are more susceptible to Pseudomonas

aeruginosa infection than males, and that they mount a stronger

inflammatory response in the lungs.

Affiliations

Departments of *Experimental Medicine and Urology, McGill University

Health Center, Montreal General Hospital Research Institute, Montreal,

Canada, Dalhousie University, Department of Physiology and Biophysics,

Halifax, Nova Scotia, Canada, §Cleveland Clinic Foundation, Department

of Immunology, Lerner Research Institute, Cleveland, OH, USA and

¶McGill University Health Center, Department of Experimental Medicine,

Meakins-Christies Laboratories, Quebec, Canada

Correspondence

Dr D. Radzioch, McGill University Health Center, Montreal General

Hospital Research Institute, 1650, Cedar Avenue, Room L11-218,

Montreal,

Quebec H3G 1A4 Canada. E-mail: danuta.radzioch@...

To cite this article

Guilbault, Claudine, Stotland, , Lachance, Claude, Tam, Mifong,

Keller, , -Snipes, Luann, Cowley, , Hamilton,

A., Eidelman, H., son, M. & Radzioch,

DanutaInfluence of gender and interleukin-10 deficiency on the

inflammatory response during lung infection with Pseudomonas

aeruginosa

in mice.Immunology 107 (3), 297-305.