Article on Ralstonia and Pandoraea Species

July 5, 2003

Journal of Clinical Microbiology, July 2003, p. 3415-3418, Vol. 41, No. 7

Use of Amplified Ribosomal DNA Restriction Analysis for Identification of

Ralstonia and Pandoraea Species: Interest in Determination of the

Respiratory Bacterial Flora in Patients with Cystic Fibrosis

Segonds,* Sandrine Paute, and Gérard Chabanon Observatoire

Cepacia/Vaincre la Mucoviscidose, Laboratoire de Bactériologie-Hygiène,

Hôpital Rangueil-TSA 50032, 31059 Toulouse Cedex 9, France Received 7

November 2002/ Returned for modification 21 January 2003/ Accepted 24

April 2003The recovery of Ralstonia and Pandoraea species from

respiratory tract cultures of patients with cystic fibrosis has recently

been reported. These species are difficult to identify, and especially to

differentiate from Burkholderia cepacia complex organisms, with classical

methods. The discriminatory power of amplified ribosomal DNA restriction

analysis (ARDRA) within the two genera was assessed by comparing the

restriction profiles of reference strains of each species by using a

panel of six enzymes already proven suitable for the identification of

Burkholderia species. ARDRA provided differentiation of all the Ralstonia

species tested and of Pandoraea norimbergensis. Pandoraea species P.

pnomenusa, P. sputorum, P. pulmonicola, and P. apista were not

discriminated to the species level. This method allowed the

identification of five clinical isolates recovered from French cystic

fibrosis patients as Ralstonia mannitolilytica. * Corresponding author.

Mailing address: Laboratoire de Bactériologie-Hygiène, Hôpital

Rangueil-TSA 50032, 31059 Toulouse Cedex 9, France. Phone:

33-(0)5-61-32-28-28. Fax: 33-(0)5-61-32-26-20. E-mail: segonds@....

Journal of Clinical Microbiology, July 2003, p. 3415-3418, Vol. 41, No. 7

0095-1137/03/$08.00+0 DOI: 10.1128/JCM.41.7.3415-3418.2003

Becki

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