Fat Stem Cells to & Type 1 Diabetes treatment

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Due to the limited supply of donor pancreas, it is imperative that we identify

alternative cell sources that can be used to treat diabetes mellitus.

Multipotent adipose tissue-derived stem cells (ADSC) can be abundantly and

safely isolated for autologous transplantation and therefore is an ideal

candidate. Here, we report the derivation of insulin-producing cells from human

or rat ADSC by transduction with the pancreatic duodenal homeobox 1 (Pdx1) gene.

RT-PCR analyses showed that native ADSC expressed insulin, glucagon, and NeuroD

genes that were upregulated following Pdx1-transduction. ELISA analyses showed

that the transduced cells secreted increasing amount of insulin in response to

increasing concentration of glucose. Transplantation of these cells under the

renal capsule of streptozotocin- induced diabetic rats resulted in lowered blood

glucose, higher glucose tolerance, smoother fur, and less cataract. Histological

examination showed that the

transplanted cells formed tissue-like structures and expressed insulin. Thus,

ADSC expressing Pdx1 appear to be suitable for treatment of diabetes mellitus.

http://www.liebertonline.com/doi/abs/10.1089/scd.2009.0010

Love, Gabby. :0)

http://stemcellforautism.blogspot.com/

 

" I know of nobody who is purely Autistic or purely neurotypical. Even God had

some Autistic moments, which is why the planets all spin. " ~ Jerry Newport