Effect of the fat composition of a single meal on the composition and cytotoxic potencies of lipolytically-releasable free fatty acids in postprandial plasma.

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Hi All,

Note the conclusions:

" Further study showed that all long-chain monounsaturated FFA and

polyunsaturated FFA, but not saturated FFA,

incorporated into lipoproteins (LDL) were cytotoxic to cultured MPM.

In conclusion, despite the generally well-accepted belief that SF is more

atherogenic than PUF, the present study

provides in vitro evidence that the lipolytic remnant products of TG-rich

lipoproteins produced after a meal rich in PUF

are more injurious to arterial wall cells than those produced after a meal rich

in SF. "

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve & db=PubMed & list_uids=9\

862181 & dopt=Abstract

Atherosclerosis 1998 Dec;141(2):321-32 Related Articles, Books, LinkOut

Effect of the fat composition of a single meal on the composition and cytotoxic

potencies of lipolytically-releasable

free fatty acids in postprandial plasma.

Chung BH, Hennig B, Cho BH, Darnell BE.

Department of Medicine, University of Alabama at Birmingham, 35294-0012, USA.

chung@...

Ingestion of a meal increases plasma levels of triglyceride (TG)-rich

lipoproteins through the secretion of

intestine-derived chylomicrons and liver-derived very low density lipoproteins

(VLDL).

We have determined the effects of the fat composition of a single meal on the

composition of TG in TG-rich lipoproteins

(VLDL + chylomicrons) and circulating and lipolytically-releasable free fatty

acids (FFA) in postprandial (PP) plasma

and on the cytotoxic potencies of the lipolytically-released FFA to cultured

arterial wall cells.

PP lipemia was induced by feeding fasted normolipidemic human subjects with a

meal rich in saturated fat (SF) and

another meal rich in polyunsaturated fat (PUF), or vice versa; each meal

provided 65% of energy as fat, and

polyunsaturated to saturated fatty acid ratios (P/S) of the SF and PUF in the

meals were 0.40 and 2.49, respectively.

The mean P/S of TG in TG-rich lipoproteins (1.43) and circulating FFA (1.46) in

4 h PP plasma of PUF were significantly

higher than those in PP plasma of SF (0.44 and 0.59, respectively) in fasting

plasma (0.52 and 0.53, respectively).

In vitro lipolysis of fasting and PP serum by purified bovine milk lipoprotein

lipase (LpL) resulted in a marked

(8.8-12.3-fold) increase in the serum FFA level.

The P/S of serum FFA in postlipolysis fasting and PP serum were consistently

higher than that of FFA or that of TG

associated with TG-rich lipoproteins in prelipolysis fasting and PP serum,

indicating that polyunsaturated TG in VLDL

and/or chylomicrons is more susceptible than saturated TG to lipolysis.

When postlipolysis serum was interacted with cultured endothelial cells and

mouse peritoneal macrophages (MPM), the

lipolytically-released FFA in PP serum of SF and PUF disrupted the barrier

function of endothelial cells and were

cytotoxic to cultured MPM; FFA in postlipolysis fasting serum was not cytotoxic.

FFA in postlipolysis PP serum of PUF were consistently more potent than that in

postlipolysis PP serum of SF.

Further study showed that all long-chain monounsaturated FFA and polyunsaturated

FFA, but not saturated FFA,

incorporated into lipoproteins (LDL) were cytotoxic to cultured MPM. In

conclusion, despite the generally well-accepted

belief that SF is more atherogenic than PUF, the present study provides in vitro

evidence that the lipolytic remnant

products of TG-rich lipoproteins produced after a meal rich in PUF are more

injurious to arterial wall cells than those

produced after a meal rich in SF.

PMID: 9862181 [PubMed - indexed for MEDLINE]

========================

Good Health & Long Life,

Greg , http://optimalhealth.cia.com.au gowatson@...

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