Occurrence of Toxigenic Aspergillus versicolor Isolates and Sterigmatocystin in Carpet Dust from Damp Indoor Environments

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http://aem.asm.org/cgi/content/abstract/68/8/3886

Applied and Environmental Microbiology, August 2002, p. 3886-3890, Vol. 68,

No. 8

0099-2240/02/$04.00+0 DOI: 10.1128/AEM.68.8.3886-3890.2002

Copyright © 2002, American Society for Microbiology. .

Occurrence of Toxigenic Aspergillus versicolor Isolates and Sterigmatocystin

in Carpet Dust from Damp Indoor Environments

Steffen Engelhart,1* Annette Loock,1 Dirk Skutlarek,1 Helmut Sagunski,2

Annette Lommel,2 Harald Färber,1 and Exner1

Institute of Hygiene and Public Health, University of Bonn, Bonn,1 Behörde

für Arbeit, Gesundheit und Soziales, Amt für Gesundheit, Freie und

Hansestadt, Hamburg, Germany2

Received 17 October 2001/ Accepted 13 May 2002

Over the past decade, there has been growing concern regarding the role of

toxigenic fungi in damp indoor environments; however, there is still a lack

of field investigations on exposure to mycotoxins. The goal of our pilot

study was to quantify the proportion of toxigenic Aspergillus versicolor

isolates in native carpet dust from damp dwellings with mold problems and to

determine whether sterigmatocystin can be detected in this matrix. Carpet

dust samples (n = 11) contained from <2.5 x 101 to 3.6 x 105 (median, 3.1 x

104) A. versicolor CFU/g of dust, and the median proportion of A. versicolor

from total culturable fungi was 18%. Based on thin-layer chromatography

detection of sterigmatocystin, 49 of 50 A. versicolor isolates (98%) were

found to be toxigenic in vitro. By using high-performance liquid

chromatography-electrospray ionization tandem mass spectrometry,

sterigmatocystin could be detected in low concentrations (2 to 4 ng/g of

dust) in 2 of 11 native carpet dust samples. From this preliminary study, we

conclude that most strains of A. versicolor isolated from carpet dust are

able to produce sterigmatocystin in vitro and that sterigmatocystin may

occasionally occur in carpet dust from damp indoor environments. Further

research and systematic field investigation are needed to confirm our

results and to provide an understanding of the health implications of

mycotoxins in indoor environments.

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* Corresponding author. Mailing address: Institute of Hygiene and Public

Health, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.

Phone: 49-228-287-5520. Fax: 49-228-287-5645. E-mail:

steffen.engelhart@....

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Applied and Environmental Microbiology, August 2002, p. 3886-3890, Vol. 68,

No. 8

0099-2240/02/$04.00+0 DOI: 10.1128/AEM.68.8.3886-3890.2002

Copyright © 2002, American Society for Microbiology. .