Proteolytic enzimes

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It is confusing topic..according to some researchers, enzymes do not

facilitate metastases...but in mice...

karla

Mixture of trypsin, chymotrypsin and papain reduces formation of metastases and

extends survival time of C57Bl6 mice with syngeneic melanoma B16.

Wald M, Olejár T, Sebková V, Zadinová M, Boubelík M, Poucková P.

Department of Surgery, 2nd Medical School, University, Prague, Czech

Republic. martin.wald@...

PURPOSE: The aim of the present study was to investigate the effect of a mixture

of proteolytic enzymes (comprising trypsin, chymotrypsin and papain) on the

metastatic model of syngeneic melanoma B16. METHODS: 140 C57B16 mice were

divided into two control and two " treated " groups. Control groups received

saline rectally, twice a day starting 24 h after intracutaneous transplantation

(C1) or from the time point of the primary B16 melanoma extirpation (C2),

respectively. " Treated " groups were rectally administered a mixture of 0.2 mg

trypsin, 0.5 mg papain, and 0.2 mg chymotrypsin twice daily starting 24 h after

transplantation (E1) or after extirpation of the tumor (E2), respectively.

Survival of mice and B16 melanoma generalization were observed for a period of

100 days. Immunological evaluation of B16 melanoma cells in the ascites was

accomplished. CD44, CD54 and CD106 cells were measured by flow cytometry.

RESULTS: Administration of proteolytic enzymes to mice inhibited the growth of

primary tumors, and tumor recurrences were less numerous. Importantly,

metastasis was considerably curtailed both in the vicinity of the primary tumor

and at distant locales. These findings correlated with a decreased expression of

CD44 and CD54 molecules in tumors exposed to proteolytic enzymes in vivo.

CONCLUSIONS: Our data suggest that serine and cysteine proteinases suppress B16

melanoma, and restrict its metastatic dissemination in C57B16 mice