Re: Re: Curcumin again but later

[Guest guest]

I just had to look a bit longer and I found this.

Joe C.

ABSTRACT

FULL TEXT

FULL-TEXT PDF (483 KB)

CITATION ALERT

CITED BY

RELATED ARTICLES

EXPORT CITATION

EMAIL TO A COLLEAGUE

REPRINTS/PERMISSIONS

BOOKMARK ARTICLE

FULL TEXT ELSEWHERE

Wilms' tumour gene 1 (WT1) as a target in curcumin treatment of pancreatic

cancer cells

Wolfgang Glienke, e Maute, Johannes Wicht, Lothar Bergmann

Received 10 December 2008; accepted 15 December 2008. published online 04

February 2009.

Abstract

The transcription factor WT1 plays an important role in cellular proliferation

and survival of various cancer cells, and is frequently expressed in pancreatic

cancer. Curcumin has been shown to be a potentially effective agent in

pancreatic cancer. In this context, the purpose of this study was to determine

the role of WT1 in a curcumin-treated pancreatic cancer cell line.

To study the effect of curcumin on the expression of WT1, we incubated the

pancreatic cancer cell line PANC-1 with different amounts of curcumin. The

expression of WT1 on mRNA and protein level was measured with real-time RT-PCR

and Western blot analysis. The incubation of the pancreatic cancer cell line

PANC-1 with curcuminresulted in an inhibition of cellular proliferation as

measured with MTT assay. The expression of WT1 on mRNA and protein level was

significantly down-regulated in a concentration-dependent manner after treatment

withcurcumin. The WT1 mRNA levels were decreased by 20%, 25%, 40%, 78% and 88%

in response to 10, 20, 30, 40 and 50?M curcumin. The use of small inhibitory RNA

(siRNA) targeting WT1 down-regulated the expression of WT1 about 90%. Combined

treatment with curcumin and siRNA targeting WT1 resulted in a significant

inhibition of cell proliferation compared to curcumin-treated cells alone. In

conclusion, WT1 is involved in cellular proliferation of PANC-1 cells. Targeting

WT1 gene expression with siRNA may enhance the efficacy of curcuminto inhibit

cell proliferation.