Vitamin uptake, CR and low-fat diet = an okay combination?

[Guest guest]

Hi All,

The below not PDF-available paper seems to say that fat levels do not affect

the uptake of fat-soluble vitamin E.

It seems important to eat enough foods containing lycopene and

lutein/zeaxanthin.

Cheers, Al.

Alan Pater, Ph.D.; Faculty of Medicine; Memorial University; St. 's, NL

A1B 3V6 Canada; Tel. No.: (709) 777-6488; Fax No.: (709) 777-7010; email:

apater@...

Experimental Gerontology

Article in Press, Corrected Proof

An in vitro model of caloric restriction

de Cabo^a, Stefanie Fürer-Galbán^b, R. Anson^a, 1,

Gilman^a, Myriam Gorospe, , a, b and Mark A. Lane^a, 2

Abstract

The mechanisms underlying the ability of caloric restriction (CR) to extend

life span and

enhance stress responsiveness remain elusive. Progress in this area has been

slow due to the

complexities of using animals for CR studies and assessing life span as the

measure of CR

effectiveness. It is therefore of great interest to develop in vitro models

of CR. Here we use sera

obtained from either Fisher 344 rats or Rhesus monkeys that were fed ad

libitum (AL) or CR diets

to culture various cell types. We show that treatment of cultured cells with

CR sera caused reduced

cell proliferation, enhanced tolerance to oxidants and heat, and heightened

expression of

stress-response genes. These phenotypic features mirror the effects of CR in

animals.

Supplementation of CR serum with insulin and insulin-like growth factor

(IGF)-1 partially restored

the proliferative and stress-response phenotype that was seen in cells

cultured with AL serum,

indicating that reduced levels of insulin and IGF-1 likely contribute to the

CR-related effects. This in

vitro cell culture model recapitulates key in vivo proliferative and

stress-response phenotypic

features of CR, and further suggests that endocrine mechanisms contribute to

the enhanced stress

responsiveness observed in CR animals.

Author Keywords: Stress-response; Hepatocytes; Heat shock; Oxidative stress;

Aging

2 Present address: Merck and Co. Inc., Rahway, NJ 07065, USA.